Poster

         Ethnobotany

Toward Genomic Resource for Ethnobotany of Lakota: Complete Chloroplast Genome of Artemisia ludoviciana subspecies ludoviciana  (Asteraceae)   

Presenting Author
Madhav Nepal
Description
Artemisia ludoviciana subspecies ludoviciana, commonly known as white sagebrush or prairie sage, is a perennial wild plant important to Native American communities for ceremonial and medicinal values. The chloroplast (cp) genome of the white sagebrush has not been reported to date. The main objective of this research was to sequence the chloroplast genome of A. ludoviciana subspecies ludoviciana to address taxonomic challenges among the six subspecies and within the genus as a whole.  In this study, total genomic DNA of the leaf tissue was extracted and sequenced using the 2nd generation sequencing technology. The sequencing produced over 20 GB of short clean reads. The reads were assembled and annotated. We found that the chloroplast genome of A. ludoviciana is 156,824 bp in size and includes 92 coding genes, 36 tRNAs and 7 rRNA. The genome components include the inverted repeat B (IRB) of 27,481 bp (129,344-156,824), small single copy (SSC) of 18,006 bp (111,337-129,343), inverted repeat A (IRA) region of 27,481bp (83,857-111,337) and large single copy (LSC) region of 83,856 bp (1-83856). We are currently conducting a phylogenomic analysis of all available Artemisia chloroplast genomes, and we will share the major findings on phylogenetic relationships during our presentation.